Rapid isolation of genomic DNA from E. coli XL1 Blue strain approaching bare magnetic nanoparticles

The aim of the present study was to develop a simple, rapid and inexpensive protocol of ultrapure bacterial genomic DNA extraction suitable for use in molecular methodology. This communication describes a protocol which uses superparamagnetic bare nanoparticles for isolation and purification of genomic DNA from overnight culture of Escherichia coli XL1 Blue strain. A comparison is also made with the conventional phenol–chloroform method and the commercially available DNA extraction kit. The tested method successfully yields ultrapure genomic DNA without RNA and protein contamination comparable to the extracted DNA using the commercially available kit. Additionally, the one-step magnetic method took less than 25 min to extract DNA against several hours taken by conventional protocols. Furthermore, the protocol successfully permitted the PCR amplification of a fragment of the bacterial 16S rDNA gene. The extracted DNA was also successfully digested by restriction endonuclease. The significance of this study was to establish a simple protocol for rapid isolation of PCR-ready bacterial genomic DNA.